Human Stem-Cell Vector Core
Human Stem-Cell Core
The hESC/iPSC core facility was established in 2008 within the Center for Cellular and Molecular Therapeutics at The Children’s Hospital of Philadelphia. The facility is located on the 5th floor of the new Colket Translational Research Building. Our mission is to provide expertise and quality-control reagents for the culture and differentiation of human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) to the CHOP and University of Pennsylvania academic communities. The facility maintains five of the new NIH-approved human ESC lines and several human iPSC lines. Stringent protocols are established to:
1) Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators.
2) Differentiate hESCs/iPSCs into mesoderm, endoderm and their derivative tissues under controlled, serum-free conditions with defined cytokines.
3) Generate iPSCs from human subjects.
In addition to these standard protocols, we will work with investigators to design new protocols tailored for their individualized research. Technologies for creating and manipulating ESCs/iPSCs are evolving rapidly and we are dedicated to incorporating the latest technologies and protocols into our facility infrastructure. For further information, please contact any of the following people:
Deborah L. French, PhD
Paul Gadue, PhD
Mitchell Weiss, MD, PhD
Click here to visit the Human Hematopoietic Stem Cell Center's website.