Part of The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, the Research Vector Core is designed and developed to support investigators interested in using viral vectors in their research model systems.
The Research Vector Core provides state-of-the-art technology support for investigators requiring viral-based vectors for gene transfer in basic research and pre-clinical studies.
The core utilizes molecular biology techniques and follows Good Laboratory Practice (GLP) guidelines to engineer and produce premium quality recombinant AAV and Lentiviral (LV) vectors at a variety of scales. The core works closely with investigators to develop vectors for individual experiments.
The research vector core has 2 support rooms dedicated to AAV and Lentiviral productions. The rooms contain three 6-ft hoods, 2 sets of standard double stacker incubators, and 4 roller bottle incubators (100 bottle capacity ea) in total. In adjacent laboratory space, there are equipment for manufacture, quality controls and other molecular biological works.
The specialized equipment include microfluidizer, centrifuge and ultracentrifuges with assorted rotors, large and small-scale Tangential Flow Filtration (TFF) for manufacture, incubator, shaker, microplate reader, Nanodrop, digital PCR, pH meter, thermocycler, gel electrophoresis, and western blot for quality control.